CpSAT-1, a transcribed satellite sequence from the codling moth, Cydia pomonella

0468806 - BC 2017 RIV NL eng J - Journal Article
Věchtová, Pavlína - Dalíková, Martina - Sýkorová, Miroslava - Žurovcová, Martina - Füssy, Zoltán - Zrzavá, Magda
CpSAT-1, a transcribed satellite sequence from the codling moth, Cydia pomonella.
Genetica. Roč. 144, č. 4 (2016), s. 385-395. ISSN 0016-6707. E-ISSN 1573-6857
R&D Projects: GA ČR GA523/09/2106; GA ČR(CZ) GA14-22765S
Institutional support: RVO:60077344
Keywords : Cydia pomonella * satellite DNA * holokinetic chromosomes * sex chromosomes * Lepidoptera
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.207, year: 2016

Satellite DNA (satDNA) is a non-coding component of eukaryotic genomes, located mainly in heterochromatic regions. Relevance of satDNA began to emerge with accumulating evidence of its potential yet hardly comprehensible role that it can play in the genome of many organisms. We isolated the first satDNA of the codling moth (Cydia pomonella, Tortricidae, Lepidoptera), a species with holokinetic chromosomes and a single large heterochromatic element, the W chromosome in females. The satDNA, called CpSAT-1, is located on all chromosomes of the complement, although in different amounts. Surprisingly, the satellite is almost missing in the heterochromatic W chromosome. Additionally, we isolated mRNA from all developmental stages (1st-5th instar larva, pupa, adult), both sexes (adult male and female) and several tissues (Malpighian tubules, gut, heart, testes, and ovaries) of the codling moth and showed the CpSAT-1 sequence was transcribed in all tested samples. Using CpSAT-1 specific primers we amplified, cloned and sequenced 40 monomers from cDNA and gDNA, respectively. The sequence analysis revealed a high mutation rate and the presence of potentially functional motifs, mainly in non-conserved regions of the monomers. Both the chromosomal distribution and the sequence analysis suggest that CPSAT-1 has no function in the C. pomonella genome.
Permanent Link: http://hdl.handle.net/11104/0266624