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Single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
- 1.0468793 - ÚIACH 2017 GB eng A - Abstract
Klepárník, Karel - Ledvina, Vojtěch - Janečková, Eva - Matalová, Eva
Single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells.
Congress Workbook. London, 2016.
[Single Cell Analysis Congress /4./. 10.11.2016-11.11.2016, London]
R&D Projects: GA ČR(CZ) GA14-28254S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : bioluminescence * single-cell analysis * caspase-3/7
Subject RIV: CB - Analytical Chemistry, Separation
Bioluminescent assay based on modified aminoluciferin was used for determination of apoptotic proteases caspase-3/7. A limit of detection beyond the average content in single cells was reached by using a photomultiplier with cooled photocathode working in photon counting regime and by miniaturization of the detection cell volume to 4 μl.
Permanent Link: http://hdl.handle.net/11104/0266618
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Number of the records: 1