Silymarin from Silybum marianum – new approaches to separation and derivatization

0468439 - MBÚ 2017 DE eng A - Abstract
Biedermann, David - Křenková, Alena - Valentová, Kateřina - Křen, Vladimír
Silymarin from Silybum marianum – new approaches to separation and derivatization.
Planta medica. Roč. 81, S01/2016 (2016). ISSN 0032-0943. E-ISSN 1439-0221.
[Joint Natural Products Conference 2016. 24.07.2016-27.07.2016, Kodaň]
R&D Projects: GA ČR(CZ) GA15-03037S; GA MŠMT(CZ) LD15081
Institutional support: RVO:61388971
Keywords : Milk thistle * silymarin * silybin
Subject RIV: CC - Organic Chemistry

Milk thistle (Silybum marianum) has been used medicinally from the 14th century [1]. Today it is a source of silymarin – extract of its fruits – which is used as nutraceutical and in the treatment of the liver problems. Despite high commercial value and large amount produced, until recently preparative separation of the silymarin component has not been achieved. Consequently, extensive research of its biological activity was not possible.
Silymarin was separated by the combination of Sephadex LH-20 chromatography, kinetic resolution and crystallization [2,3]. Silymarin flavonolignans were all obtained in gram quantities and high purity. The derivatization was mainly carried out on silychristin.[4] The toxicity was assayed against several cell lines of different histological origin, the cytotoxicity is mostly negligible. Radical scavenging activity and lipoperoxidation inhibition is very high (eg. anhydrosilychristin lipoperoxidation inhibition IC50=4.12 µM). This is in agreement with observed low redox potentials (dehydrosilychristin 0.39 V) [4].

Permanent Link: http://hdl.handle.net/11104/0267397