Comparison of human glutamate carboxypeptidases II and III reveals their divergent substrate specificities

0468320 - ÚOCHB 2017 RIV GB eng J - Journal Article
Navrátil, Michal - Tykvart, Jan - Schimer, Jiří - Pachl, Petr - Navrátil, Václav - Rokob, T. A. - Hlouchová, Klára - Rulíšek, Lubomír - Konvalinka, Jan
Comparison of human glutamate carboxypeptidases II and III reveals their divergent substrate specificities.
FEBS Journal. Roč. 283, č. 13 (2016), s. 2528-2545. ISSN 1742-464X. E-ISSN 1742-4658
R&D Projects: GA ČR(CZ) GBP208/12/G016; GA ČR(CZ) GA14-31419S; GA MŠMT LO1302; GA MŠMT(CZ) LO1304
Institutional support: RVO:61388963
Keywords : arene-binding site * GCPIII * prostate-specific membrane antigen * QM/MM calculations * beta-citryl-L-glutamate
Subject RIV: CE - Biochemistry
Impact factor: 3.902, year: 2016

Glutamate carboxypeptidase III (GCPIII) is best known as a homologue of glutamate carboxypeptidase II [GCPII; also known as prostate-specific membrane antigen (PSMA)], a protease involved in neurological disorders and overexpressed in a number of solid cancers. However, mouse GCPIII was recently shown to cleave beta-citrylglutamate (BCG), suggesting that these two closely related enzymes have distinct functions. To develop a tool to dissect, evaluate and quantify the activities of human GCPII and GCPIII, we analysed the catalytic efficiencies of these enzymes towards three physiological substrates. We observed a high efficiency of BCG cleavage by GCPIII but not GCPII. We also identified a strong modulation of GCPIII enzymatic activity by divalent cations, while we did not observe this effect for GCPII. Additionally, we used X-ray crystallography and computational modelling (quantum and molecular mechanical calculations) to describe the mechanism of BCG binding to the active sites of GCPII and GCPIII, respectively. Finally, we took advantage of the substantial differences in the enzymatic efficiencies of GCPII and GCPIII towards their substrates, using enzymatic assays for specific detection of these proteins in human tissues. Our findings suggest that GCPIII may not act merely as a complementary enzyme to GCPII, and it more likely possesses a specific physiological function related to BCG metabolism in the human body.
Permanent Link: http://hdl.handle.net/11104/0266128