Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples

0468000 - ÚIACH 2017 RIV CZ eng C - Conference Paper (international conference)
Kubáň, Pavel - Boček, Petr - Seip, K. F. - Gjelstad, A. - Pedersen-Bjergaard, S.
Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2016 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 158-161. ISBN 978-80-904959-4-4.
[CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./. Brno (CZ), 17.10.2016-19.10.2016]
R&D Projects: GA ČR(CZ) GA16-09135S
Institutional support: RVO:68081715
Keywords : micro-electromembrane extraction * sample pretreatment techniques * biological samples
Subject RIV: CB - Analytical Chemistry, Separation

Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic
solutions was developed for selective extractions of various analytes from untreated
biological samples. The novel approach requires μL volumes of samples and organic
solvents, which form consecutive immiscible plugs in a transparent polymeric capillary
and the extraction process is accelerated by application of d.c. voltage. Number of the
plugs, their composition, volume and sequence in the extraction capillary can be chosen
specifically for each particular application and high variability and selectivity of the
extraction process can be achieved. In this contribution, we demonstrate suitability of
a five-phase μ-EME system for simultaneous extractions of cations and anions from
urine and for selective extractions of basic analytes based on their acid-base strength
from plasma.
Permanent Link: http://hdl.handle.net/11104/0265923