0467891 - MBÚ 2017 RIV NL eng J - Journal Article
Cheel, José - Bogdanová, K. - Ignatova, S. - Garrard, I. - Hewitson, P. - Kolář, M. - Kopecký, Jiří - Hrouzek, Pavel - Vacek, J.
Dimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCC.
Algal Research-Biomass Biofuels and Bioproducts. Roč. 18, SEP (2016), s. 244-249. ISSN 2211-9264. E-ISSN 2211-9264
R&D Projects: GA MŠMT EE2.3.30.0059; GA MŠMT ED2.1.00/03.0110; GA MŠMT(CZ) LO1416
Institutional support: RVO:61388971
Keywords : Phenolic cyclopentenedione * Natural antibiotic * Cyanobacteria
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.994, year: 2016 ; AIS: 0.969, rok: 2016
DOI: https://doi.org/10.1016/j.algal.2016.06.022

The need for new antimicrobial agents is greater than ever because of the emergence of multidrug resistance in common pathogens and incidence of new infections. Cyclopent-4-ene-1,3-diones (CPDs) have been reported as a new class of compounds with promising antimicrobial and antifungal properties. Herein we report the selective antibiotic properties of nostotrebin 6, a phenolic CPD produced biotechnologically by the culture of cyanobacterium Nostoc sp. str. Lukesova 27/97. High performance countercurrent chromatography (HPCCC) combined with gel permeation chromatography (GPC) was used for the isolation of nostotrebin 6 with a relatively high 0.53 +/- 0.1% yield (calculated from dried biomass) and final purity higher than 96%. Nostotrebin 6 was tested for its antimicrobial and antifungal activities by using standard micro-dilution method, and the results were expressed as minimal inhibitory concentrations (MICs). Nostotrebin 6 unequivocally inhibited the growth of Gram-positive reference (Enterococcus faecalis CCM 4224, Staphylococcus aureus CCM 4223 and Staphylococcus aureus CCM 3953) and multidrug-resistant (Staphylococcus haemolyticus A/16568, Staphylococcus aureus MRSA 4591 and Enterococcus faecium VanA 419/ana) strains. Its strongest effect was exerted against the Gram-positive bacteria with MICs ranging between 6.25 and 15.6 mu g/mL. There was no effect on Gram-negative strains tested and yeasts. Our results suggest that nostotrebin 6 could serve as basic nucleus for further design of novel antibiotic agents and demonstrate that the bio-production approach based on HPCCC/GPC isolation endpoint is an efficient methodology for obtaining nostotrebin 6 in multi-gram scale. Furthermore, the presented isolation method can be easily up-scaled to process kilograms of the cyanobacterial biomass.
Permanent Link: http://hdl.handle.net/11104/0266187