Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells

0467739 - ÚIACH 2017 RIV CZ eng C - Conference Paper (international conference)
Ledvina, Vojtěch - Klepárník, Karel
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2016 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 170-173. ISBN 978-80-904959-4-4.
[CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./. Brno (CZ), 17.10.2016-19.10.2016]
R&D Projects: GA ČR(CZ) GA14-28254S
Institutional support: RVO:68081715
Keywords : single-cell analysis * apoptotic cells * non-apoptotic cells
Subject RIV: CB - Analytical Chemistry, Separation

Caspases are proteases that play key role in the process of apoptosis, the programmed
cell death. Among them, caspase-3 and -7 are main executioner caspases that cleave
many vital proteins during apoptosis and after their widespread activation, the process
cannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized
device for parallel analysis of eight samples was developed. The assay is based on the
modified luciferin-firefly luciferase bioluminescence (BL) system. Individual
suspended cells were collected and transferred into detection microvials using a
micromanipulator. The bioluminescence was detected using a photon counting head
with cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both
apoptotic and non-apoptotic cells was reached.
Permanent Link: http://hdl.handle.net/11104/0265776