Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry

0467709 - ÚIACH 2017 RIV CZ eng C - Conference Paper (international conference)
Flodrová, Dana - Toporová, L. - Macejová, D. - Laštovičková, Markéta - Brtko, J. - Bobálová, Janette
Comparative evaluation of protein composition in human breast cancer cells using mass spectrometry.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2016 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 114-117. ISBN 978-80-904959-4-4.
[CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./. Brno (CZ), 17.10.2016-19.10.2016]
Grant - others:Akademie věd - GA AV ČR(CZ) SAV-15-01
Program: Bilaterální spolupráce
Institutional support: RVO:68081715
Keywords : mass spectrometry * breast cancer * human tumour * bottom-up proteomic approach
Subject RIV: CB - Analytical Chemistry, Separation

Bottom-up proteomic approach was used for detailed characterization of proteins from
two human tumour cell lines representing major clinically different types of breast
cancer. The aim was to show the differences between them on proteomic level. Here
we present almost 100 unequivocally identified proteins out of which 60 were mutually
differently expressed for MDA-MB-231 and MCF-7. Some well-known breast cancer
markers like annexins A1, A2 and vimentin were found in the MDA-MB-231 cell line.
On the other hand, MCF-7 cells were found to be positive for cytokeratins and keratins
and thus we were able to distinguish both cell lines sufficiently.
Permanent Link: http://hdl.handle.net/11104/0265753