CE-nESI/MS in electrode-free design: narrowing the separation channel to 5 μm

0467705 - ÚIACH 2017 RIV CZ eng C - Conference Paper (international conference)
Týčová, Anna - Foret, František
CE-nESI/MS in electrode-free design: narrowing the separation channel to 5 μm.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2016 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 261-265. ISBN 978-80-904959-4-4.
[CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./. Brno (CZ), 17.10.2016-19.10.2016]
R&D Projects: GA ČR(CZ) GBP206/12/G014; GA ČR(CZ) GA15-15479S
Institutional support: RVO:68081715
Keywords : CE-nESI/MS * electrode-free arragement
Subject RIV: CB - Analytical Chemistry, Separation

Capillary electrophoresis-nanospray/mass spectrometry (CE-nESI/MS) in electrodefree
arrangement represents a very simple instrumentation for analysis of biomolecules.
So far, CE-nESI/MS analyses in this design were limited to 10-75 μm ID of the
separation channel. Although the work with narrower dimensions brings several
challenges, there is a great potential for better sensitivity, improved separation power
and lower sample consumption. This work is devoted to some practical aspects of
experiments in narrow bore channels and systematic evaluation of CE-nESI/MS
analyses conducted in capillaries of 25, 15 and 5 μm ID.
Permanent Link: http://hdl.handle.net/11104/0265752