Characterization of FRET sensor

0467703 - ÚIACH 2017 RIV CZ eng C - Conference Paper (international conference)
Datinská, Vladimíra - Klepárník, Karel - Belšánová, B. - Minárik, M. - Foret, František
Characterization of FRET sensor.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2016 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 103-105. ISBN 978-80-904959-4-4.
[CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./. Brno (CZ), 17.10.2016-19.10.2016]
R&D Projects: GA TA ČR(CZ) TA02010672; GA ČR(CZ) GA14-28254S
Institutional support: RVO:68081715
Keywords : FRET sensor * Fӧrster resonance energy transfer * quantum dot
Subject RIV: CB - Analytical Chemistry, Separation

In this study, we present characterization of sensor based on Fӧrster resonance energy
transfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory
synthesized quantum dot (QD). A complementary chain of a sample is labeled by a
luminescent dye. When the dsDNA hybrid is formed, the energy from the QD (donor)
is transferred to the dye (acceptor) and FRET is observed as a decrease of QD
luminescence emission intensity and an increase of dye luminescence emission
intensity.
Permanent Link: http://hdl.handle.net/11104/0265749