Number of the records: 1  

The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware

  1. 1.
    0467641 - MBÚ 2017 RIV US eng J - Journal Article
    Weiszenstein, M. - Pavlíková, N. - Elkalaf, M. - Halada, Petr - Šeda, O. - Trnka, J. - Kovář, J. - Polák, J.
    The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware.
    PLoS ONE. Roč. 11, č. 3 (2016), e0152382. ISSN 1932-6203. E-ISSN 1932-6203
    R&D Projects: GA MŠMT(CZ) LO1509
    Institutional support: RVO:61388971
    Keywords : ADIPOSE-TISSUE * GLUTAMINE-METABOLISM * RISK-FACTOR
    Subject RIV: EE - Microbiology, Virology
    Impact factor: 2.806, year: 2016

    Pericellular oxygen concentration represents an important factor in the regulation of cell functions, including cell differentiation, growth and mitochondrial energy metabolism. Hypoxia in adipose tissue has been associated with altered adipokine secretion profile and suggested as a possible factor in the development of type 2 diabetes. In vitro experiments provide an indispensable tool in metabolic research, however, physical laws of gas diffusion make prolonged exposure of adherent cells to desired pericellular O-2 concentrations questionable. The aim of this study was to investigate the direct effect of various O-2 levels (1%, 4% and 20% O-2) on the proteomic profile and triglyceride accumulation in 3T3-L1 differentiated preadipocytes using gas-permeable cultureware. Following differentiation of cells under desired pericellular O-2 concentrations, cell lysates were subjected to two-dimensional gel electrophoresis and protein visualization using Coomassie blue staining. Spots showing differential expression under hypoxia were analyzed using matrix-assisted laser desorption/ionization mass spectrometry. All identified proteins were subjected to pathway analysis. We observed that protein expression of 26 spots was reproducibly affected by 4% and 1% O-2 (17 upregulated and 9 downregulated). Pathway analysis showed that mitochondrial energy metabolism and triglyceride synthesis were significantly upregulated by hypoxia. In conclusion, this study demonstrated the direct effects of pericellular O-2 levels on adipocyte energy metabolism and triglyceride synthesis, probably mediated through the reversed tricarboxylic acid cycle flux.
    Permanent Link: http://hdl.handle.net/11104/0266223

     
     
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.