Identification and characterization of multiple emissive species in aggregated minor antenna complexes

0467590 - MBÚ 2017 RIV NL eng J - Journal Article
Wahadoszamen, M. - Belgio, Erica - Rahman, M.A. - Ara, A.M. - Ruban, A.V. - van Grondelle, R.
Identification and characterization of multiple emissive species in aggregated minor antenna complexes.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1857, č. 12 (2016), s. 1917-1924. ISSN 0005-2728. E-ISSN 1879-2650
Institutional support: RVO:61388971
Keywords : Light harvesting * Minor antenna complexes * Photoprotective energy dissipation
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.932, year: 2016

Aggregation induced conformational change of light harvesting antenna complexes is believed to constitute one of the pathways through which photosynthetic organisms can safely dissipate the surplus of energy while exposed to saturating light. In this study, Stark fluorescence (SF) spectroscopy is applied to minor antenna complexes (CP24, CP26 and CP29) both in their light-harvesting and energy-dissipating states to trace and characterize different species generated upon energy dissipation through aggregation (in-vitro) induced conformational change. SF spectroscopy could identify three spectral species in the dissipative state of CP24, two in CP26 and only one in CP29. The comprehensive analysis of the SF spectra yielded different sets of molecular parameters for the multiple spectral species identified in CP24 or CP26, indicating the involvement of different pigments in their formation. Interestingly, a species giving emission around the 730 nm spectral region is found to form in both CP24 and CP26 following transition to the energy dissipative state, but not in CP29. The SF analyses revealed that the far red species has exceptionally large charge transfer (CT) character in the excited state. Moreover, the far red species was found to be formed invariably in both Zeaxanthin (Z)- and Violaxathin (V)-enriched CP24 and CP26 antennas with identical CT character but with larger emission yield in Z -enriched ones. This suggests that the carotenoid Z is not directly involved but only confers an allosteric effect on the formation of the far red species.
Permanent Link: http://hdl.handle.net/11104/0266225