Kapilární separace, mikrofluidika a hmotnostní spektrometrie pro bioanalýzu

0467152 - ÚIACH 2017 CZ eng A - Abstract
Foret, František - Křenková, Jana - Klepárník, Karel - Týčová, Anna - Partyka, Jan
Kapilární separace, mikrofluidika a hmotnostní spektrometrie pro bioanalýzu.
Sborník přednášek a posterů XXV. Biochemický sjezd. Praha: Česká společnost pro biochemii a molekulární biologii, 2016 - (Pačes, V.; Stiborová, M.; Turňa, J.)
[Biochemický sjezd /25./. 13.09.2016-16.09.2016, Praha]
Institutional support: RVO:68081715
Keywords : microfabricated liquid junction * electrospray tip * electrospray ionization
Subject RIV: CB - Analytical Chemistry, Separation

In this work we have explored two, application dependent, approaches. A hybrid design, utilizing a microfabricated liquid junction/sprayer module, allowed coupling to a commercial, fully automated, CE analyser equipped with standard (50-75 m ID) separation capillaries. In the second, “interface-free” approach, the CE/MS analysis was performed in a narrow bore (<20 m ID) separation capillary forming one entity with the electrospray tip. The high voltage, applied at the injection end of the capillary, served for both the separation and electrospray ionization. Optimum conditions for the separation and electrospray ionization were achieved with voltage programming. The performance of both CE/MS systems was tested for separations of metabolites, peptides and glycoproteins and compared to other designs with respect to the separation efficiency, sensitivity and sample consumption.
Permanent Link: http://hdl.handle.net/11104/0265273