Polycyclic aromatic hydrocarbons degradation and microbial community shifts during co-composting of creosote-treated wood

0466645 - MBÚ 2017 RIV NL eng J - Journal Article
Covino, Stefano - Fabiánová, Tereza - Křesinová, Zdena - Čvančarová, Monika - Burianová, Eva - Filipová, Alena - Voříšková, Jana - Baldrian, Petr - Cajthaml, Tomáš
Polycyclic aromatic hydrocarbons degradation and microbial community shifts during co-composting of creosote-treated wood.
Journal of Hazardous Materials. Roč. 301, JAN 15 (2016), s. 17-26. ISSN 0304-3894. E-ISSN 1873-3336
R&D Projects: GA MŠMT(CZ) EE2.3.30.0003; GA TA ČR TE01020218; GA ČR GA13-28283S
Institutional support: RVO:61388971
Keywords : Composting * Bioremediation * Polycyclic aromatic hydrocarbons
Subject RIV: EE - Microbiology, Virology
Impact factor: 6.065, year: 2016

The feasibility of decontaminating creosote-treated wood (CPA) by co-composting with agricultural wastes was investigated using two bulking agents, grass cuttings (GC) and broiler litter (BL), each employed at a 1:1 ratio with the matrix. The initial concentration of total polycyclic aromatic hydrocarbons (PAHs) in CTW (26,500 mg kg(-1)) was reduced to 3 and 19% after 240 d in GC and BL compost, respectively. PAH degradation exceeded the predicted bioaccesible threshold, estimated through sequential supercritical CO2 extraction, together with significant detoxification, assessed by contact tests using Vibrio fisheri and Hordeum vulgare.

GC composting was characterized by high microbial biomass growth in the early phases, as suggested by phospholipid fatty acid analyses. Based on the 454-pyrosequencing results, fungi (mostly Saccharomycetales) constituted an important portion of the microbial community, and bacteria were characterized by rapid shifts (from Firmicutes (Bacilli) and Actinobacteria to Proteobacteria). However, during BL composting, larger amounts of prokaryotic and eukaryotic PLFA markers were observed during the cooling and maturation phases, which were dominated by Proteobacteria and fungi belonging to the Ascomycota and those putatively related to the Glomeromycota. This work reports the first in-depth analysis of the chemical and microbiological processes that occur during the co-composting of a PAH-contaminated matrix.
Permanent Link: http://hdl.handle.net/11104/0265371