Late-stage alpha-synuclein accumulation in TNWT-61 mouse model of Parkinson's disease detected by diffusion kurtosis imaging

0466604 - ÚPT 2017 RIV US eng J - Journal Article
Khairnar, A. - Rudá-Kučerová, J. - Dražanová, Eva - Szabó, N. - Latta, P. - Arab, A. - Hutter-Paier, B. - Havas, D. - Windisch, M. - Šulcová, A. - Starčuk jr., Zenon - Király, A. - Rektorová, I.
Late-stage alpha-synuclein accumulation in TNWT-61 mouse model of Parkinson's disease detected by diffusion kurtosis imaging.
Journal of Neurochemistry. Roč. 136, č. 6 (2016), s. 1259-1269. ISSN 0022-3042. E-ISSN 1471-4159
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : diffusion kurtosis imaging * diffusion kurtosis imaging * Parkinson's disease * TBSS * TNWT-61 * transgenic mice
Subject RIV: BM - Solid Matter Physics ; Magnetism
Impact factor: 4.083, year: 2016

Diffusion kurtosis imaging (DKI) by measuring non-Gaussian diffusion allows an accurate estimation of the distribution of water molecule displacement and may correctly characterize microstructural brain changes caused by neurodegeneration. The aim of this study was to evaluate the ability of DKI to detect changes induced by -synuclein (-syn) accumulation in -syn over-expressing transgenic mice (TNWT-61) in both gray matter (GM) and white matter (WM) using region of interest (ROI) and tract-based spatial statistics analyses, respectively, and to explore the relationship between -syn accumulation and DKI metrics in our regions of interest. Fourteen-month-old TNWT-61 mice and wild-type (WT) littermates underwent invivo DKI scanning using the Bruker Avance 9.4 Tesla magnetic resonance imaging system. ROI analysis in the GM regions substantia nigra, striatum, hippocampus, sensorimotor cortex, and thalamus and tract-based spatial statistics analysis in WM were performed. Immunohistochemistry for -syn was performed in TNWT-61 mice and correlated with DKI findings. We found increased kurtosis and decreased diffusivity values in GM regions such as the thalamus and sensorimotor cortex, and in WM regions such as the external and internal capsule, mamillothalamic tract, anterior commissure, cingulum, and corpus callosum in TNWT-61 mice as compared to WT mice. Furthermore, we report for the first time that -syn accumulation is positively correlated with kurtosis and negatively correlated with diffusivity in the thalamus. The study provides evidence of an association between the amount of -syn and the magnitude of DKI metric changes in the ROIs, with the potential of improving the clinical diagnosis of Parkinson's disease.
Permanent Link: http://hdl.handle.net/11104/0264875