Method of surface modification for analysing phosphorylated peptides

0465798 - MBÚ 2017 RIV eng P - Patent Document
Volný, Michael - Novák, Petr - Havlíček, Vladimír - Pompach, Petr
Method of surface modification for analysing phosphorylated peptides.
2016. Owner: Mikrobiologický ústav AV ČR, v. v. i. Date of the patent acceptance: 07.06.2016. Patent Number: US9358573
R&D Projects: GA ČR GPP206/10/P018; GA ČR(CZ) GD204/09/H084; GA MŠMT(CZ) ME10013; GA ČR(CZ) GAP206/12/1150; GA MŠMT(CZ) LO1509
Institutional support: RVO:61388971
Keywords : phosphorelation * maldi * FTICR
Subject RIV: EE - Microbiology, Virology
http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&co1=AND&d=PTXT&s1=9358573&OS=9358573&RS=9358573

The present invention relates to a method of making modification of solid substrates for efficient surface preconcentration of phosphorylated peptides from complex peptide mixtures prior detection based on desorption-ionization mass spectrometry. The subject of the protection is a method of modifying surfaces used as substrates for desorption-ionization mass spectrometry. The method according to the present invention is based on electronebulization (electrospraying) of a solution of organometallic compounds of the group 4B elements, and namely zirconium, hafnium and titanium. The charged elecrospray thus obtained is dried in real time by passage through a heated space and impinges onto a surface to which it fixedly binds in the form of oxide.; The surface modified in such a manner can then be used for preconcentration of phosphorylated peptides from peptide mixtures with subsequent analysis using desorption-ionization mass spectrometry.
Permanent Link: http://hdl.handle.net/11104/0264294