The method of surface modification for the purpose of enrichment of phosphorylated peptides for analysis by desorption/ionization mass spectrometry techniques

0465769 - MBÚ 2017 RIV eng P - Patent Document
Volný, Michael - Novák, Petr - Havlíček, Vladimír - Pompach, Petr
The method of surface modification for the purpose of enrichment of phosphorylated peptides for analysis by desorption/ionization mass spectrometry techniques.
2015. Owner: Mikrobiologický ústav AV ČR, v. v. i. Date of the patent acceptance: 25.11.2015. Patent Number: EP2652769
R&D Projects: GA ČR GPP206/10/P018; GA ČR(CZ) GD204/09/H084; GA MŠMT(CZ) ME10013; GA ČR(CZ) GAP206/12/1150; GA MŠMT(CZ) LO1509
Institutional support: RVO:61388971
Keywords : phosphorelation * maldi * FTICR
Subject RIV: EE - Microbiology, Virology
https://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20151125&DB=&locale=en_EP&CC=EP&NR=2652769B1&KC=B1&ND=4

The present invention relates to a method of making modification of solid substrates for efficient surface preconcentration of phosphorylated peptides from complex peptide mixtures prior detection based on desorption-ionization mass spectrometry. The subject of the protection is a method of modifying surfaces used as substrates for desorption-ionization mass spectrometry. The method according to the present invention is based on electronebulization (electrospraying) of a solution of organometallic compounds of the group 4B elements, and namely zirconium, hafnium and titanium. The charged elecrospray thus obtained is dried in real time by passage through a heated space and impinges onto a surface to which it fixedly binds in the form of oxide.; The surface modified in such a manner can then be used for preconcentration of phosphorylated peptides from peptide mixtures with subsequent analysis using desorption-ionization mass spectrometry.
Permanent Link: http://hdl.handle.net/11104/0264297