The deposition of inhaled titanium nanoparticles in mice organs

0465385 - ÚPT 2017 IE eng A - Abstract
Machala, M. - Kulich, P. - Šerý, O. - Marvanová, S. - Skoupý, Radim - Rusnák, A. - Mikuška, Pavel - Večeřa, Zbyněk
The deposition of inhaled titanium nanoparticles in mice organs.
Toxicology Letters. Elsevier. Roč. 258, Supplement 16 SEP (2016), S277. ISSN 0378-4274. E-ISSN 1879-3169.
[EUROTOX. Congress of the European Societies of Toxicology /52./. 04.09.2016-07.09.2016, Seville]
Institutional support: RVO:68081731 ; RVO:68081715
Keywords : nanoparticles * deposition of titanium
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering; CE - Biochemistry (UIACH-O)

In the present study, biokinetics and deposition of titanium
dioxide nanoparticles were studied in female ICR mice, after continuous 12-week exposure in inhalation chamber, using transmission electron microscopy (TEM), scan electron microscopy (SEM) and theSEMcoupled with energy dispersed spectroscopy system (EDX). Uniformity, shape and size of NPs were characterized by TEM and SEM. The study particularly focused on the distribution of Ti NPs in selected tissues. The samples of lung, liver, kidney, spleen and brain were sectioned, fixed and then embedded in Epon–Durcupan mixture. TEM and/or SEM were used for sample observation. Finally, EDX was used in order to evaluate Ti presence in secondary lysosomes of target organ cells. The results indicate that Ti NPs may pass into alveoli and then passively transfer through their membrane, as no signs of phagocytosis or endocytosis were observed. The exposure to Ti NPs gradually induced a loss of type I pneumocytes
and alveoli thickening. Within the type II pneumocytes,
Ti NPs were found to be deposited within secondary lysosomes, as
confirmed by two types of independent EDX analyses. In general,
our findings seem to support the hypothesis that the inhaled Ti NPs
are translocated via lung-red blood cells-target organ axis and that
erythrocytes may serve as principle carriers of Ti NPs.
Permanent Link: http://hdl.handle.net/11104/0263984