In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM

0465246 - ÚPT 2017 RIV GB eng C - Conference Paper (international conference)
Neděla, Vilém - Bučko, M. - Tihlaříková, Eva - Krajčovič, T. - Gemeiner, P. - Navrátilová, Eva - Hudec, Jiří
In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM.
EMC2016. The 16th European Microscopy Congress. Proceedings. Oxford: Wiley, 2016, s. 263-264. ISBN 9783527808465.
[EMC2016. European Microscopy Congress /16./. Lyon (FR), 28.08.2016-02.09.2016]
R&D Projects: GA TA ČR(CZ) TE01020118; GA ČR(CZ) GA14-22777S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : ESEM * morphology * polyelectrolyte complex capsules
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
http://onlinelibrary.wiley.com/doi/10.1002/9783527808465.EMC2016.5932/pdf

Polyelectrolyte complex (PEC) capsules/beads are very important for biotechnological applications such as drug delivery and bacterial whole-cell biocatalyst development. The very beam-sensitive bio-polymer capsules are laboratory produced as a uniform with a controlled shape, size, membrane thickness, permeability and mechanical resistance. PEC capsules are very sensitive to any treatment and samples could be inspected in their fully native and functional state to prevent any misinterpretation. Characterization and study of PEC capsules properties is possible using thermodynamically stabile and fully wet state, precisely reached after very slow changing of conditions in the specimen chamber of ESEM. The morphological study using low current ESEM was already presented. The internal structure can be in solvent, semisolid or solid state, depend on capsule type and manufacturing process, nevertheless it was not described in its native state yet. Study of inner part as well as surface morphology of PEC capsules using classical SEM or cryo-SEM can be misleading due to requirement of dry resp. freeze sample. The aim of this work is in-situ study of internal structure of PEC capsules in fully wet state and demonstration of state of matter of PEC capsules core.
Permanent Link: http://hdl.handle.net/11104/0263896