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A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics
- 1.0465045 - ÚPT 2017 RIV US eng J - Journal Article
Tacke, S. - Krzyžánek, Vladislav - Nüsse, H. - Wepf, R.A. - Klingauf, J. - Reichelt, R.
A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.
Biophysical Journal. Roč. 110, č. 4 (2016), s. 758-765. ISSN 0006-3495. E-ISSN 1542-0086
R&D Projects: GA ČR(CZ) GA14-20012S
Institutional support: RVO:68081731
Keywords : transmission electron microscopy * cryoelectron tomography * cells
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 3.656, year: 2016
Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.
Permanent Link: http://hdl.handle.net/11104/0263768
Number of the records: 1