A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway

0464656 - MBÚ 2017 RIV DE eng J - Journal Article
Parajuli, A. - Kwak, D.H. - Dalponte, L. - Leikoski, N. - Galica, Tomáš - Umeobika, U. - Trembleau, L. - Sivonen, K. - Wahlsten, M. - Wang, H. - Rizzi, E. - De Bellis, G. - Naismith, J. - Jaspars, M. - Liu, X.Y. - Houssen, W. - Fewer, D.P.
A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway.
Angewandte Chemie - International Edition. Roč. 55, č. 11 (2016), s. 3596-3599. ISSN 1433-7851. E-ISSN 1521-3773
R&D Projects: GA MŠMT(CZ) LO1416
Institutional support: RVO:61388971
Keywords : biosynthesis * cyanobactins * peptides
Subject RIV: EE - Microbiology, Virology
Impact factor: 11.994, year: 2016

Cyanobactins are a rapidly growing family of linear and cyclic peptides produced by cyanobacteria. KawaguchipeptinsA and B, two macrocyclic undecapeptides reported earlier from Microcystis aeruginosa NIES-88, are shown to be products of the cyanobactin biosynthetic pathway. The 9kb kawaguchipeptin (kgp) gene cluster was identified in a 5.26Mb draft genome of Microcystis aeruginosa NIES-88. We verified that this gene cluster is responsible for the production of the kawaguchipeptins through heterologous expression of the kgp gene cluster in Escherichia coli. The KgpF prenyltransferase was overexpressed and was shown to prenylate C-3 of Trp residues in both linear and cyclic peptides invitro. Our findings serve to further enhance the structural diversity of cyanobactins to include tryptophan-prenylated cyclic peptides.
Permanent Link: http://hdl.handle.net/11104/0263746