Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms

0464651 - MBÚ 2017 RIV NL eng J - Journal Article
Řezanka, Tomáš - Nedbalová, L. - Sigler, Karel
Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms.
Journal of Chromatography A. Roč. 1467, October (2016), s. 261-269. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR GA14-00227S
Institutional support: RVO:61388971
Keywords : Chiral triacylglycerols * Enantiomers * Regioisomers
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.981, year: 2016

Regioisomers and enantiomers of triacylglycerols (TAGs) containing any combination of stearidonic (18:4n-3) and octadecapentaenoic (18:5n-3) acids were prepared by organic synthesis. Gradient polar organic liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-LC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from the haptophyte alga Coccolithophora sp. cultivated in a salinity range from 7.5 to 60 parts per thousand. The ratio of regioisomers and enantiomers was found to change with increasing salinity of the culture medium. This can be explained by variable activity of acyltransferases in cells exposed to salt stress. (C) 2016 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0263747