Plant Cytogenetics

0464631 - ÚEB 2017 RIV US eng M - Monography Chapter
Šafář, Jan - Šimková, Hana - Doležel, Jaroslav
Construction of BAC libraries from flow-sorted chromosomes.
Plant Cytogenetics. Vol. 1429. New York: Humana Press, 2016 - (Kianian, S.; Kianian, P.), s. 135-149. Methods and Protocols, Methods in Molecular Biology. ISBN 978-1-4939-3620-5
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : BAC library * BAC vector * Chromosomes
Subject RIV: EB - Genetics ; Molecular Biology

Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes.
Permanent Link: http://hdl.handle.net/11104/0263463