Plant Cytogenetics

0464540 - ÚEB 2017 RIV US eng M - Monography Chapter
Karafiátová, Miroslava - Bartoš, Jan - Doležel, Jaroslav
Localization of low-copy DNA sequences on mitotic chromosomes by FISH.
Plant Cytogenetics. Vol. 1429. New York: Humana Press, 2016 - (Kianian, S.; Kianian, P.), s. 49-64. Methods and Protocols, Methods in Molecular Biology. ISBN 978-1-4939-3620-5
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : Cell cycle synchronization * Cytogenetic mapping * Fluorochrome
Subject RIV: EB - Genetics ; Molecular Biology

Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contributed to numerous studies and important discoveries. Over the decades, the protocol was modified for ease of use, allowing for localizing multiple probes simultaneously and increasing its sensitivity and specificity. Despite the continuous improvements, the ability to detect short single-copy sequences of only a few kilobases or less, such as genes, remains limited. Here, we provide a detailed protocol for detection of short, single- or low-copy sequences on plant mitotic metaphase chromosomes.
Permanent Link: http://hdl.handle.net/11104/0263409