From the Tunnels into the Treetops: New Lineages of Black Yeasts from Biofilm in the Stockholm Metro System and Their Relatives among Ant-Associated Fungi in the Chaetothyriales

0464147 - BÚ 2017 RIV US eng J - Journal Article
Réblová, Martina - Hubka, Vít - Thureborn, O. - Lundberg, J. - Sallstedt, T. - Wedin, M. - Ivarsson, M.
From the Tunnels into the Treetops: New Lineages of Black Yeasts from Biofilm in the Stockholm Metro System and Their Relatives among Ant-Associated Fungi in the Chaetothyriales.
PLoS ONE. Roč. 11, č. 10 (2016), s. 1-36, č. článku e0163396. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GAP506/12/0038
Grant - others:GA MŠk(CZ) CZ.1.05/1.1.00/02.0109
Institutional support: RVO:67985939 ; RVO:61388971
Keywords : Chaetothyriales * molecular phylogenetics * speleothem biofilm
Subject RIV: EF - Botanics; EE - Microbiology, Virology (MBU-M)
Impact factor: 2.806, year: 2016

Isolation of fungi from speleothem biofilm covering bare granite walls in the Kungsträdgården metro station in Stockholm yielded axenic cultures of two distinct black yeast morphotypes. Phylogenetic analyses of DNA sequences from six nuclear loci, ITS, nuc18S and nuc28S rDNA, rpb1, rpb2 and β-tubulin, support their placement in the Chaetothyriales (Ascomycota). They are described as a new genus Bacillicladium with the type species B. lobatum, and a new species Bradymyces graniticola. Bacillicladium is distantly related to the known five chaetothyrialean families and is unique in the Chaetothyriales by variable morphology showing hyphal, meristematic and yeast-like growth in vitro. The genus Bradymyces is placed amidst members of the Trichomeriaceae and is ecologically undefined; it includes an opportunistic animal pathogen while two other species inhabit rock surfaces. Compensatory base change criterion in the ITS2 secondary structure supported delimitation of species in Bradymyces, which manifest a limited number of phenotypic features useful for species recognition.
Permanent Link: http://hdl.handle.net/11104/0265031