Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells

0463697 - ÚIACH 2017 RIV DE eng J - Journal Article
Ledvina, Vojtěch - Janečková, Eva - Matalová, Eva - Klepárník, Karel
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells.
Analytical and Bioanalytical Chemistry. Roč. 409, č. 1 (2017), s. 269-274. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA ČR(CZ) GA14-28254S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : single-cell analysis * bioluminescence * apoptosis * caspase-3/7
OECD category: Analytical chemistry; Developmental biology (UZFG-Y)
Impact factor: 3.307, year: 2017

The analyses of chemical content of individual cells have already proved the capability to bring unique information on various biological processes. Single-cell analysis provides more accurate and reliable results for biology and medicine than analyses of extracts from cell populations, where a natural heterogeneity is averaged. To meet the requirements in the research of important biologically active molecules, such as caspases, we have developed a miniaturized device for simultaneous analyses of individual cells.
Permanent Link: http://hdl.handle.net/11104/0262805