Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae

0463694 - MBÚ 2017 RIV NL eng J - Journal Article
Moudříková, Š. - Mojzeš, P. - Zachleder, Vilém - Pfaff, Ch. - Behrendt, D. - Nedbal, L.
Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae.
Algal Research-Biomass Biofuels and Bioproducts. Roč. 16, June (2016), s. 224-232. ISSN 2211-9264. E-ISSN 2211-9264
Institutional support: RVO:61388971
Keywords : Biofuels * Lipids * Photosynthesis
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.994, year: 2016

Chlorophylls and carotenoids are core components of photosynthetic energy transduction in algal cells, whereas starch, lipids, and polyphosphates represent energy reserves. All these biomolecules exhibit characteristic molecular vibrations that were sensed and localized in individual cells of Desmodesmus quadricauda and Chlorella vulgaris by confocal Raman microscopy. In the same cells, fluorescence of chlorophylls and of lipid bodies stained with Nile Red was mapped using the same instrument. In the first of the three consecutive scans, a low-power and short-exposure excitation by a green 532 nm laser was used to map chlorophyll fluorescence. In the second scan, the full power of a 785 nm laser was used for Raman mapping of carotenoids.
Permanent Link: http://hdl.handle.net/11104/0263053