Vibrational and electronic circular dichroism as powerful tools for the conformational analysis of cationic antimicrobial peptides

0463448 - ÚOCHB 2017 RIV AT eng J - Journal Article
Kocourková, L. - Novotná, P. - Šťovíčková-Habartová, L. - Čujová, Sabína - Čeřovský, Václav - Urbanová, M. - Setnička, V.
Vibrational and electronic circular dichroism as powerful tools for the conformational analysis of cationic antimicrobial peptides.
Monatshefte fur Chemie. Roč. 147, č. 8 (2016), s. 1439-1445. ISSN 0026-9247. E-ISSN 1434-4475
Institutional support: RVO:61388963
Keywords : conformation * circular dichroism * antimicrobial peptides * liposomes * infrared spectroscopy
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.282, year: 2016

Antimicrobial and hemolytic activities of cationic alpha-helical antimicrobial peptides depend on their ability to adopt an amphipathic alpha-helical conformation on the cell membrane surface. Using vibrational and electronic circular dichroism, we carried out a conformational study of two alpha-helical antimicrobial peptides, melectin and antapin, in various environments mimicking bacterial and eukaryotic membranes. The results showed a significant difference in the content of alpha-helical conformation in the environment mimicking the bacterial and eukaryotic membranes.
Permanent Link: http://hdl.handle.net/11104/0262641