CD46 and β1 integrin dynamics during sperm acrosome reaction

0463158 - BTÚ 2017 FR eng C - Conference Paper (international conference)
Dvořáková-Hortová, Kateřina - Frolíková, Michaela - Děd, Lukáš - Šebková, Nataša
CD46 and β1 integrin dynamics during sperm acrosome reaction.
Indian Journal of Psychiatry. ISSN 0019-5545. E-ISSN 1998-3794.
[19th European tetsis Workshop - Eurpoean Workshop on the molecular and cellular endocrinology of the testis. Saint-Malo (FR), 11.06.2016-15.06.2016]
R&D Projects: GA ČR GA14-05547S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : CD46 * β1-integrin * sperm capacitation * acrosome reaction * STED
Subject RIV: EC - Immunology

CD46 půays an important role during fertilization. It is localized in the acrosome membrane and its role is associated with acrosomal stability. CD46 is probably involved in signaling pathways triggering AR.Failure of CD46 expression is suspected to alter intracellular signaling and disrupt the acrosome region due to affecting a distribution of integrins and lately actin. It also associates, through membrane integrins, with specific MAP kinases involved in the AR. Integrins interact with many cytoskeletal proteins, therefore changes in the actin cytoskeleton before and after AR may lead to changes in the association and localization of CD46 and β1-integrin. The interaction of these proteins in sper can be predicted, however it still has not been shown. It was documented that essential sperm-egg fusion protein IZUMO1 changes their localization during the AR towards the place where sperm-egg fusion happens. This process is made possible by enzyme Tssk6 which activity affects actin cytoskeleton and itsrole in the transport of sperm proteins into the postacrosome region of the sperm head. The question remains, whether CD46 and β1-integrin changes its localization during the acrosome reaction and whether this possible change is affected by the actin cytoskeleton. Faulty restructuring of these proteins throughout the capacitation may therefore, results in unsuccessful fusion of acrosomal membrane at the beggining of the AR.
Permanent Link: http://hdl.handle.net/11104/0262877