Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction

0463058 - BTÚ 2017 RIV GB eng J - Journal Article
Frolíková, Michaela - Šebková, Nataša - Děd, Lukáš - Dvořáková-Hortová, Kateřina
Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction.
Scientific Reports. Roč. 6, Sep 26 (2016), s. 1-15. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR GA14-05547S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : CD46 * β1 integrin * acrosome reaction * actin * SIM * latrunculin A
Subject RIV: EC - Immunology
Impact factor: 4.259, year: 2016

The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It is widely recognized that specific sperm proteins form an active network prior to fertilization, and their dynamic relocation is crucial for the sperm-egg fusion. The unique presence of the membrane cofactor protein CD46 in the sperm acrosomal membrane was shown, however, its behaviour and connection with other sperm proteins has not been explored further. Using super resolution microscopy, we demonstrated a dynamic CD46 reorganisation over the sperm head during the AR, and its interaction with transmembrane protein integrins, which was confirmed by proximity ligation assay. Furthermore, we propose their joint involvement in actin network rearrangement. Moreover, CD46 and β1 integrins with subunit α3, but not α6, are localized into the apical acrosome and are expected to be involved in signal transduction pathways directing the acrosome stability and essential protein network rearrangements prior to gamete fusion.
Permanent Link: http://hdl.handle.net/11104/0262898