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Stratification of yeast cells during chronological aging by size points to the role of trehalose in cell vitality
- 1.0462514 - MBÚ 2017 RIV US eng J - Journal Article
Švenkrtová, Andrea - Belicová, Lenka - Volejníková, Andrea - Sigler, Karel - Jazwinski, S.M. - Pichová, Alena
Stratification of yeast cells during chronological aging by size points to the role of trehalose in cell vitality.
Biogerontology. Roč. 17, č. 2 (2016), s. 395-408. ISSN 1389-5729. E-ISSN 1573-6768
R&D Projects: GA MŠMT LH13049
Institutional support: RVO:61388971
Keywords : STATIONARY-PHASE CULTURES * SACCHAROMYCES-CEREVISIAE * LIFE-SPAN
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.231, year: 2016
Cells of the budding yeast Saccharomyces cerevisiae undergo a process akin to differentiation during prolonged culture without medium replenishment. Various methods have been used to separate and determine the potential role and fate of the different cell species. We have stratified chronologically-aged yeast cultures into cells of different sizes, using centrifugal elutriation, and characterized these subpopulations physiologically. We distinguish two extreme cell types, very small (XS) and very large (L) cells. L cells display higher viability based on two separate criteria. They respire much more actively, but produce lower levels of reactive oxygen species (ROS).
Permanent Link: http://hdl.handle.net/11104/0261962
Number of the records: 1