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Calcium-Driven Folding of RTX Domain beta-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts
- 1.0462041 - MBÚ 2017 RIV US eng J - Journal Article
Bumba, Ladislav - Mašín, Jiří - Macek, Pavel - Wald, Tomáš - Motlová, Lucia - Bíbová, Ilona - Klímová, Nela - Bednárová, Lucie - Veverka, Václav - Kachala, M. - Svergun, D. I. - Bařinka, Cyril - Šebo, Peter
Calcium-Driven Folding of RTX Domain beta-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts.
Molecular Cell. Roč. 62, č. 1 (2016), s. 47-62. ISSN 1097-2765. E-ISSN 1097-4164
R&D Projects: GA MŠMT(CZ) LK11205; GA MŠMT(CZ) LO1304; GA ČR(CZ) GA15-11851S; GA ČR GA13-14547S; GA ČR GAP302/12/0460; GA ČR(CZ) GAP207/11/0717; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971 ; RVO:61388963 ; RVO:86652036
Keywords : ADENYLATE-CYCLASE TOXIN * GRAM-NEGATIVE BACTERIA * BORDETELLA-PERTUSSIS
Subject RIV: CE - Biochemistry; EB - Genetics ; Molecular Biology (BTO-N); CE - Biochemistry (UOCHB-X)
Impact factor: 14.714, year: 2016
Calcium-binding RTX proteins are equipped with C-terminal secretion signals and translocate from the Ca2+-depleted cytosol of Gram-negative bacteria directly into the Ca2+-rich external milieu, passing through the "channel-tunnel" ducts of type I secretion systems (T1SSs). Using Bordetella pertussis adenylate cyclase toxin, we solved the structure of an essential C-terminal assembly that caps the RTX domains of RTX family leukotoxins. This is shown to scaffold directional Ca2+-dependent folding of the carboxy-proximal RTX repeat blocks into beta-rolls. The resulting intramolecular Brownian ratchets then prevent backsliding of translocating RTX proteins in the T1SS conduits and thereby accelerate excretion of very large RTX leukotoxins from bacterial cells by a vectorial "push-ratchet" mechanism.
Permanent Link: http://hdl.handle.net/11104/0261587
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