HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells

0462034 - BFÚ 2017 RIV US eng J - Journal Article
Kudová, Jana - Procházková, J. - Vašíček, Ondřej - Perečko, Tomáš - Sedláčková, M. - Pešl, M. - Pachernik, J. - Kubala, Lukáš
HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
PLoS ONE. Roč. 11, č. 6 (2016). ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA13-29358S
Grant - others:Grantová agentura ČR - GA ČR(CZ) GJ15-13443Y
Institutional support: RVO:68081707
Keywords : INDUCIBLE FACTOR 1-ALPHA * GENE-EXPRESSION * CARDIAC DIFFERENTIATION
Subject RIV: BO - Biophysics
Impact factor: 2.806, year: 2016

Cardiac cell formation, cardiomyogenesis, is critically dependent on oxygen availability. It is known that hypoxia, a reduced oxygen level, modulates the in vitro differentiation of pluripotent cells into cardiomyocytes via hypoxia inducible factor-1alpha (HIF-1 alpha)-dependent mechanisms. However, the direct impact of HIF-1 alpha deficiency on the formation and maturation of cardiac-like cells derived from mouse embryonic stem cells (mESC) in vitro remains to be elucidated. In the present study, we demonstrated that HIF-1 alpha deficiency significantly altered the quality and quantity of mESC-derived cardiomyocytes. It was accompanied with lower mRNA and protein levels of cardiac cell specific markers (myosin heavy chains 6 and 7) and with a decreasing percentage of myosin heavy chain alpha and beta, and cardiac troponin T-positive cells. As to structural aspects of the differentiated cardiomyocytes, the localization of contractile proteins (cardiac troponin T, myosin heavy chain alpha and beta) and the organization of myofibrils were also different. Simultaneously, HIF-1 alpha deficiency was associated with a lower percentage of beating embryoid bodies.
Permanent Link: http://hdl.handle.net/11104/0261600