The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes

0462022 - MBÚ 2017 RIV DE eng J - Journal Article
Pavlíková, N. - Weiszenstein, M. - Pala, J. - Halada, Petr - Šeda, O. - Elkalaf, M. - Trnka, J. - Kovář, J. - Polak, J.
The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes.
Cellular & Molecular Biology Letters. Roč. 20, č. 5 (2015), s. 919-936. ISSN 1425-8153. E-ISSN 1689-1392
Institutional support: RVO:61388971
Keywords : Cell culture * Proteomics * Cultureware surface
Subject RIV: CE - Biochemistry
Impact factor: 1.753, year: 2015

Experiments using cultured primary cells or cell lines are a routine in vitro approach used across multiple biological disciplines, However, the structural and functional influences of various cultureware materials on cultured cells is not clearly understood. Surface treatments of cultureware have proven to have profound effects on cell viability and proliferation. In this study, we investigated the impact of polystyrene and fluorocarbon cultureware dishes on the proteomic profile of differentiated 3T3-L1 preadipocytes. After expansion and differentiation of cells on appropriate cultureware dishes, cell lysates were separated using two- dimensional gel electrophoresis and proteins were visualized with Coomassie blue staining. Spots with the highest differential expression between the two culture conditions were subsequently analyzed using matrix- assisted laser desorption/ionization mass spectrometry and the identified proteins were subjected to pathway analysis.
Permanent Link: http://hdl.handle.net/11104/0261550