Expression of human beta-N-acetylhexosaminidase B in yeast eases the search for selective inhibitors

0461990 - MBÚ 2017 RIV US eng J - Journal Article
Krejzová, Jana - Kulik, Natalia - Slámová, Kristýna - Křen, Vladimír
Expression of human beta-N-acetylhexosaminidase B in yeast eases the search for selective inhibitors.
Enzyme and Microbial Technology. Roč. 89, JUL (2016), s. 1-6. ISSN 0141-0229. E-ISSN 1879-0909
R&D Projects: GA ČR(CZ) GC15-02578J
Institutional support: RVO:61388971
Keywords : beta-N-acetylhexosaminidase * Hex B * enzyme inhibition
Subject RIV: CE - Biochemistry
Impact factor: 2.502, year: 2016

Human lysosomal beta-N-acetylhexosaminidases from the family 20 of glycoside hydrolases are dimeric enzymes catalysing the cleavage of terminal beta-N-acetylglucosamine and beta-N-acetylgalactosamine residues from a broad spectrum of glycoconjugates. Here, we present a facile, robust, and cost-effective extracellular expression of human beta-N-acetylhexosaminidase B in Pichia pastoris KM71H strain. The prepared Hex B was purified in a single step with 33% yield obtaining 10 mg of the pure enzyme per 1 L of the culture media.
Permanent Link: http://hdl.handle.net/11104/0261529