Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

0461980 - BC 2017 RIV US eng J - Journal Article
Nebesářová, Jana - Wandrol, P. - Vancová, Marie
Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy.
Nanomedicine: Nanotechnology, Biology and Medicine. Roč. 12, č. 1 (2016), s. 105-517. ISSN 1549-9634. E-ISSN 1549-9642
R&D Projects: GA TA ČR(CZ) TE01020118
Institutional support: RVO:60077344
Keywords : multiple immunolabeling * gold nanoparticles * high resolution SEM * STEM imaging * BSE imaging
Subject RIV: EA - Cell Biology
Impact factor: 5.720, year: 2016

We present a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals.
Permanent Link: http://hdl.handle.net/11104/0261517