Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug

0461865 - FGÚ 2017 RIV NL eng J - Journal Article
Ujčíková, Hana - Vošahlíková, Miroslava - Roubalová, Lenka - Svoboda, Petr
Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug.
Journal of Proteomics. Roč. 145, Aug 11 (2016), s. 11-23. ISSN 1874-3919. E-ISSN 1876-7737
R&D Projects: GA ČR(CZ) GBP304/12/G069; GA ČR(CZ) GAP207/12/0919
Institutional support: RVO:67985823
Keywords : morphine * long-term exposure * rat forebrain cortex * post-nuclear supernatant * MALDI-TOF MS/MS * MaxLFQ
Subject RIV: CE - Biochemistry
Impact factor: 3.914, year: 2016

Proteomic analysis was performed in post-nuclear supernatant fraction (PNS) prepared from forebrain cortex of rats exposed to increasing doses of morphine (10–50 mg/kg) for 10 days and sacrificed 24 h (group + M10) or 20 days (group + M10/−M20) after the last dose of morphine. PNS fraction was resolved by 2D-ELFO and stained by CBB. Analysis of the difference between (+ M10) and (− M10) samples of PNS by PDQuest accompanied by MALDI-TOF MS/MS indicated the significant change of 28 proteins. Importantly, the number of altered proteins was decreased to 14 after 20 days of nurturing animals in the absence of morphine. This new and important finding indicating the ability of mammalian organism to return to physiological norm after removal of the drug was verified by an independent methodology — gel-free & label-free quantification and normalization procedure denominated as MaxLFQ. The 113 proteins were identified as altered by morphine in (+ M10) samples when compared with (− M10) samples of PNS and this number was decreased to 19 after 20 days of nurturing the animals in the absence of this drug.
Permanent Link: http://hdl.handle.net/11104/0261429