Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots

0461676 - ÚEB 2017 RIV NL eng J - Journal Article
Jiskrová, E. - Novák, Ondřej - Pospíšilová, H. - Holubová, K. - Karády, M. - Galuszka, P. - Robert, S. - Frébort, I.
Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots.
New Biotechnology. Roč. 33, č. 5 (2016), s. 735-742. ISSN 1871-6784. E-ISSN 1876-4347
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : SOLID-PHASE EXTRACTION * MAIZE BETA-GLUCOSIDASE * ARABIDOPSIS-THALIANA
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.813, year: 2016

The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.
Permanent Link: http://hdl.handle.net/11104/0261269