Modified Nucleic Acids

0461380 - ÚOCHB 2017 RIV CH eng M - Monography Chapter
Daďová, Jitka - Cahová, Hana - Hocek, Michal
Polymerase Synthesis of Base-Modified DNA.
Modified Nucleic Acids. Cham: Springer, 2016 - (Nakatani, K.; Tor, Y.), s. 123-144. Nucleic Acids and Molecular Biology, 31. ISBN 978-3-319-27109-5
Institutional support: RVO:61388963
Keywords : enzyme amplification reaction * cross-coupling reactions * labeled nucleoside triphosphonates
Subject RIV: CC - Organic Chemistry

Enzymatic synthesis of base-modified DNA by polymerase incorporation of modified nucleotides is discussed. Modified 20-deoxyribonucleoside triphosphates (dNTPs) are key substrates for polymerases and can be prepared either by triphosphorylation of modified nucleosides or by direct aqueous cross-coupling reactions of halogenated dNTPs with alkynes, arylboronic acids, or alkenes. The methods of polymerase synthesis include primer extension, PCR, nicking enzyme amplifications, and other methods which enable the synthesis of diverse types of long or short and double-stranded DNA or single-stranded oligonucleotides. The applications include labeling in diagnostics (labeling or coding of DNA bases) and chemical biology (bioconjugations, modulation of protein binding, etc.).
Permanent Link: http://hdl.handle.net/11104/0260996