Caspases and osteogenic markers-in vitro screening of inhibition impact

0460713 - ÚŽFG 2017 RIV US eng J - Journal Article
Adamová, Eva - Janečková, Eva - Klepárník, Karel - Matalová, Eva
Caspases and osteogenic markers-in vitro screening of inhibition impact.
In Vitro - Cellular and Developmental Biology. Animal Section. Roč. 52, č. 2 (2016), s. 144-148. ISSN 1071-2690. E-ISSN 1543-706X
R&D Projects: GA ČR(CZ) GB14-37368G; GA ČR(CZ) GA14-28254S
Institutional support: RVO:67985904 ; RVO:68081715
Keywords : osteogenesis * chondrogenesis * caspases * caspase-3 * gene expression
Subject RIV: EA - Cell Biology; CB - Analytical Chemistry, Separation (UIACH-O)
Impact factor: 0.897, year: 2016

Caspases, well-known players in apoptosis or inflammation, appear to have roles also in other processes such as cell differentiation. Caspase-3, in particular, was recently demonstrated to have non-apoptotic functions in osteogenesis. However, the molecular pathways involved are not yet known. Therefore, we used osteogenic PCR arrays to provide a comprehensive screening of possible interactions of caspases in general and specifically of caspase-3 in osteogenic networks. Embryonic micromass cultures derived from mouse forelimbs were established and pharmacological fluoromethylketone (FMK) inhibitors applied. Alterations were observed in expression of several genes after caspase inhibition (Bmp1, Bmp5, Bmp6, Col10a1, Col2a1, Comp, Egf, Fgfr2, Gli1, Igf1, Nog, Phex, Sox9, Spp1). The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies.
Permanent Link: http://hdl.handle.net/11104/0260736