Principal component analysis of Raman spectroscopy data for determination of biofilm forming bacteria and yeasts

0460214 - ÚPT 2017 RIV CZ eng C - Conference Paper (international conference)
Šiler, Martin - Samek, Ota - Bernatová, Silvie - Mlynariková, K. - Ježek, Jan - Šerý, Mojmír - Krzyžánek, Vladislav - Hrubanová, Kamila - Holá, M. - Růžička, F. - Zemánek, Pavel
Principal component analysis of Raman spectroscopy data for determination of biofilm forming bacteria and yeasts.
Proceedings of the 15th International Seminar on Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Brno: Institute of Scientific Instruments CAS, 2016 - (Mika, F.), s. 66-67. ISBN 978-80-87441-17-6.
[International Seminar on Recent Trends in Charged Particle Optics and Surface Physics Instrumentation /15./. Skalský dvůr (CZ), 29.05.2016-03.06.2016]
R&D Projects: GA ČR(CZ) GA15-20645S; GA ČR(CZ) GA16-12477S; GA MŠMT(CZ) LO1212
Institutional support: RVO:68081731
Keywords : electron microscopy * biofilm * Raman spectra
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
http://www.trends.isibrno.cz/

Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascular
replacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.
A method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. The
presented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.
Permanent Link: http://hdl.handle.net/11104/0260346