A two-dimensional protein map of Pleurotus ostreatus microsomes-proteome dynamics

0460130 - MBÚ 2017 RIV CZ eng J - Journal Article
Petráčková, Denisa - Halada, Petr - Bezoušková, Silvia - Křesinová, Zdena - Svobodová, Kateřina
A two-dimensional protein map of Pleurotus ostreatus microsomes-proteome dynamics.
Folia Microbiologica. Roč. 61, č. 1 (2016), s. 63-71. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA ČR(CZ) GA15-02328S
Institutional support: RVO:61388971
Keywords : FUNGUS * EXPRESSION * BIODEGRADATION
Subject RIV: DK - Soil Contamination ; De-contamination incl. Pesticides
Impact factor: 1.521, year: 2016

Recent studies documented that several processes in filamentous fungi are connected with microsomal enzyme activities. In this work, microsomal subproteomes of Pleurotus ostreatus were analyzed by two-dimensional (2-D) polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. To assess proteome dynamics, microsomal proteins were isolated from fungal cultures after 7 and 12 days of cultivation. Additionally, 10 mg/L of 17 alpha-ethinylestradiol (EE2) was treated with the cultures during 2 days. Despite the EE2 degradation by the fungus reached 97 and 76.3 % in 7- and 12-day-old cultures, respectively, only a minor effect on the composition of microsomal proteins was observed. The changes in protein maps related to ageing prevailed over those induced by EE2. Epoxide hydrolase, known to metabolize EE2, was detected in 12-day-old cultures only which suggests differences in EE2 degradation pathways utilized by fungal cultures of different age. The majority (32 %) of identified microsomal proteins were parts of mitochondrial energy metabolism.
Permanent Link: http://hdl.handle.net/11104/0260268