Nanospray interfacing for capillary electrophoresis-mass spectrometry

0459940 - ÚIACH 2017 IT eng A - Abstract
Foret, František - Křenková, Jana - Týčová, Anna - Klepárník, Karel
Nanospray interfacing for capillary electrophoresis-mass spectrometry.
Abstract book 40th ISCC and 13th GC×GC Symposium. Messina: Chromaleont S.r.L, 2016 - (Mondello, L.; Dugo, P.). s. 117-117. ISBN 978-88-941816-0-9.
[ISCC /40./ and GC×GC Symposium /13./. 29.05.2016-03.06.2016, Riva del Garda]
Institutional support: RVO:68081715
Keywords : CE/MS * microfabricated liquid junction/sprayer module * electrospray ionization * separation capillaries
Subject RIV: CB - Analytical Chemistry, Separation

Capillary electrophoresis coupled to mass spectrometry (CE/MS) is gaining its space among the most powerful tools in modern (bio)analytical laboratory. The most challenging instrumental aspect in CE/MS is striking the balance between the stability and reproducibility of the signal and sensitivity of the analysis. Several interface designs have been developed in the past decade addressing the variety of instrumental aspects and ease of operation. Most of the interfaces can be categorized either into the sheath flow arrangement (considered to be a de facto standard), or a sheathless arrangement, often expected to provide the ultimate sensitivity. In this work we have explored two, application dependent, approaches. A hybrid design, utilizing a microfabricated liquid junction/sprayer module, allowed coupling to a commercial, fully automated, CE analyser equipped with standard (50-75 um ID) separation capillaries. In the second, “interface-free” approach, the CE/MS analysis was performed in a narrow bore (um ID) separation capillary forming one entity with the electrospray tip. The high voltage, applied at the injection end of the capillary, served for both the separation and electrospray ionization. Optimum conditions for the separation and electrospray ionization were achieved with voltage programming. The performance of both CE/MS systems was tested for separations of metabolites, peptides and glycoproteins and compared to other designs with respect to the separation efficiency, sensitivity and sample consumption.
Permanent Link: http://hdl.handle.net/11104/0260099