The effect of tetrabrombisphenol A on Sertoli cells: in vivo and in vitro approach.\n\n

0459807 - BTÚ 2017 CZ eng A - Abstract
Žatecká, Eva - Děd, Lukáš - Elzeinová, Fatima - Margaryan, Hasmik - Dvořáková-Hortová, Kateřina - Pěknicová, Jana
The effect of tetrabrombisphenol A on Sertoli cells: in vivo and in vitro approach.

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Book of abstract XXIInd Symposium of immunology and biology of Reproduction with international participation. Vestec u Prahy: Biotechnologický ústav, 2016 - (Pěknicová, J.; Kubátová, A.; Elzeinová, F.). s. 30-30
[XXIInd Symposium of Immunology and Biology of Reproduction with international participation. 26.05.2016-28.05.2016, Třešť]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA ČR(CZ) GAP503/12/1834
Institutional support: RVO:86652036
Keywords : tetrabromobisphenol A * Sertoli cells * histological analysis * TUNEL analysis
Subject RIV: EB - Genetics ; Molecular Biology

Tetrabromobisphenol A (TBBPA) belongs to the most widely used flame retardants. It is found in the environment and can also be detected in the human body. Several studies have shown that TBBPA is able to negatively influenced reproductive parameters in vivo. Moreover, it has been proven that TBBPA has negative effect on different mammalian cell lines in vitro and it is able to interfere with the thyroid hormone system as well as it might be able to affect the androgen/estrogen hormone system.
In this study, we decided to examine the potential effect of TBBPA on Sertoli cells (SC) in vivo as well as in vitro. In our in vivo study, animals were exposed to TBBPA (35 ug/kg b.w.) continuously during life. In 10 weeks old animals, we showed that TBBPA is able to induce apoptosis of testicular cells, which involved mainly the diploid cell population (including SC). We also analysed expression of Sox 9 gene (SC marker), which was significantly lower in experimental group. Moreover, histological analysis revealed significantly lower thickness of seminiferous epithelium in exposed animals. Further, we focused on 4 week old (pre-pubertal) animals in order to analyse SC and their maturation status. In these animals we detected higher number of TUNEL positive cells as well as less Wt1 (SC marker) positive cells. Furthermore, we detected significantly decreased expression of genes for Wt1 and Thyroid hormone receptor, which is sensitive indicator of SC maturational state. Taken together our results suggest that TBBPA causes premature maturation of SC, which in turn leads to apoptosis of these cells. However, more experiments are needed to further support our hypothesis. Moreover, in vitro outcome of various TBBPA concentrations on SC cell line proliferation and apoptotic status showed negative concentration-dependent effect of TBBPA on proliferative activity of SC.



Permanent Link: http://hdl.handle.net/11104/0259987