From Functionalized Nanostructures Towards Engineered Macrostructures

0459258 - ÚEM 2017 RIV CZ eng M - Monography Chapter
Kubinová, Šárka - Zajícová, Alena - Holáň, Vladimír - Syková, Eva
Nanofibers as Scaffolds for Cell Transfer in Tissue Regeneration.
From Functionalized Nanostructures Towards Engineered Macrostructures. Jindřichův Hradec: Epika, 2015 - (Caruana, C.; Horník, J.), s. 82-98. ISBN 978-80-88113-20-1
R&D Projects: GA MŠMT(CZ) LO1309
Institutional support: RVO:68378041
Keywords : nanofiber * electrospinning * scaffold * stem cell * cell adhesion
Subject RIV: FP - Other Medical Disciplines

Nanofibrous materials that mimic the native extracellular matrix and promote the adhesion of various cells are being developed as tissue engineered scaffolds for the skin, bone, vasculature, heart, cornea, nervous system, and other tissues. As a new approach, nanofibers prepared from gelatin, PA6/12, PAN, PCL, PLA and PUR using industrial scale needleless technology have been introduced as potential cell carriers and evaluated in vitro using human mesenchymal stem cells (hMSC) and mouse limbal stem cells (mLSC). A viability assay as well as immunocytochemical examination revealed that hMSC as well as mLSC adhered, grew and proliferated on all tested nanofibers. This study confirmed that examined nanofibers have a promising potential for biomedical applications such as scaffolds for cell transfer in tissue regeneration.
Permanent Link: http://hdl.handle.net/11104/0259495