Early differences in metabolic flexibility between obesity-resistant and obesity-prone mice

0459062 - FGÚ 2017 RIV FR eng J - Journal Article
Bardová, Kristina - Horáková, Olga - Janovská, Petra - Hansíková, Jana - Kůs, Vladimír - van Schothorst, E. M. - Hoevenaars, F.P.M. - Uil, M. - Hensler, Michal - Keijer, J. - Kopecký, Jan
Early differences in metabolic flexibility between obesity-resistant and obesity-prone mice.
Biochimie. Roč. 124, May (2016), s. 163-170. ISSN 0300-9084. E-ISSN 1638-6183
R&D Projects: GA MŠMT(CZ) 7E10059; GA ČR(CZ) GB14-36804G
Institutional support: RVO:67985823
Keywords : indirect calorimetry * glucose tolerance * weaning * C57BL/6J mice * A/J mice * metabolic flexibility
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 3.112, year: 2016

Decreased metabolic flexibility, i.e. a compromised ability to adjust fuel oxidation to fuel availability supports development of adverse consequences of obesity. The aims of this study were (i) to learn whether obesity-resistant A/J and obesity-prone C57BL/6J mice differ in their metabolic flexibility right after weaning; and (ii) to characterize possible differences in control of glucose homeostasis in these animals using glucose tolerance tests (GTT). A/J and C57BL/6J mice of both genders were maintained at 20 degrees C and weaned to standard low-fat diet at 30 days of age. During the first day after weaning, using several separate animal cohorts, (i) GTT was performed using 1 or 3 mg glucose/g body weight (BW), while glucose was administered either orally (OGTT) or intraperitoneally (IPGTT) at 20 degrees C; and (ii) indirect calorimetry (INCA) was performed, either in a combination with oral gavage of 1 or 7.5 mg glucose/g BW, or during a fasting/re-feeding transition. INCA was conducted either at 20 degrees C or 34 degrees C. Results of both OGTT and IPGTT using 1 mg glucose/g BW at 20 degrees C, and INCA using 7.5 mg glucose/g BW at 34 degrees C, indicated higher glucose tolerance and higher metabolic flexibility to glucose, respectively, and lower fasting glycemia in A/J mice as compared with C57BL/6J mice. Thus, control of whole body glucose metabolism between A/J and C57BL/6J mice represents a phenotypic feature differentiating between the strains right after weaning.
Permanent Link: http://hdl.handle.net/11104/0259318