The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns.

0459060 - ÚEM 2017 RIV US eng J - Journal Article
Čejka, Čestmír - Holáň, Vladimír - Trošan, Peter - Zajícová, Alena - Javorková, Eliška - Čejková, Jitka
The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns.
Oxidative Medicine and Cellular Longevity. Roč. 2016, Jan (2016), s. 5843809. ISSN 1942-0900. E-ISSN 1942-0994
R&D Projects: GA ČR(CZ) GA14-12580S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LO1309; GA MŠMT(CZ) LO1508
Institutional support: RVO:68378041
Keywords : extracellular-superoxide dismutase * oxidative stress * rabbit cornea
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.593, year: 2016

The aim of this study was to examine whether mesenchymal stem cells (MSCs) and/or corneal limbal epithelial stem cells (LSCs) influence restoration of an antioxidant protective mechanism in the corneal epithelium and renewal of corneal optical properties changed after alkali burns. The injured rabbit corneas (with 0.25N NaOH) were untreated or treated with nanofiber scaffolds free of stem cells, with nanofiber scaffolds seeded with bone marrow MSCs (BM-MSCs), with adipose tissue MSCs (Ad-MSCs), or with LSCs. On day 15 following the injury, after BM-MSCs or LSCs nanofiber treatment (less after Ad-MSCs treatment) the expression of antioxidant enzymes was restored in the regenerated corneal epithelium and the expressions of matrix metalloproteinase 9 (MMP9), inducible nitric oxide synthase (iNOS), alpha-smooth muscle actin (alpha-SMA), transforming growth factor-beta1 (TGF-beta1), and vascular endothelial factor (VEGF) were low. The central corneal thickness (taken as an index of corneal hydration) increased after the injury and returned to levels before the injury. In injured untreated corneas the epithelium was absent and numerous cells revealed the expressions of iNOS, MMP9, alpha-SMA, TGF-beta1, and VEGF. In conclusion, stem cell treatment accelerated regeneration of the corneal epithelium, restored the antioxidant protective mechanism, and renewed corneal optical properties.
Permanent Link: http://hdl.handle.net/11104/0259331