iBodies: Modular Synthetic Antibody Mimetics Based on Hydrophilic Polymers Decorated with Functional Moieties

0458826 - ÚOCHB 2017 RIV DE eng J - Journal Article
Šácha, Pavel - Knedlík, Tomáš - Schimer, Jiří - Tykvart, Jan - Parolek, Jan - Navrátil, Václav - Dvořáková, Petra - Sedlák, František - Ulbrich, Karel - Strohalm, Jiří - Majer, Pavel - Šubr, Vladimír - Konvalinka, Jan
iBodies: Modular Synthetic Antibody Mimetics Based on Hydrophilic Polymers Decorated with Functional Moieties.
Angewandte Chemie - International Edition. Roč. 55, č. 7 (2016), s. 2356-2360. ISSN 1433-7851. E-ISSN 1521-3773
R&D Projects: GA ČR GBP208/12/G016; GA MŠMT LO1302
Institutional support: RVO:61388963 ; RVO:61389013
Keywords : antibody mimetics * HPMA * molecular recognition * polymer conjugates * protein targeting
Subject RIV: CE - Biochemistry; CD - Macromolecular Chemistry (UMCH-V)
Impact factor: 11.994, year: 2016
http://onlinelibrary.wiley.com/doi/10.1002/anie.201508642/full

Antibodies are indispensable tools for biomedicine and anticancer therapy. Nevertheless, their use is compromised by high production costs, limited stability, and difficulty of chemical modification. The design and preparation of synthetic polymer conjugates capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, immunocytochemistry, and immunoprecipitation is reported. The conjugates, named " iBodies", consist of an HPMA copolymer decorated with low-molecular-weight compounds that function as targeting ligands, affinity anchors, and imaging probes. We prepared specific conjugates targeting several proteins with known ligands and used these iBodies for enzyme inhibition, protein isolation, immobilization, quantification, and live-cell imaging. Our data indicate that this highly modular and versatile polymer system can be used to produce inexpensive and stable antibody substitutes directed toward virtually any protein of interest with a known ligand.
Permanent Link: http://hdl.handle.net/11104/0259057