Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity

0458566 - ÚMCH 2017 RIV HR eng J - Journal Article
Zasońska, Beata Anna - Líšková, A. - Kuricová, M. - Tulinská, J. - Pop-Georgievski, Ognen - Čiampor, F. - Vávra, I. - Dušinská, M. - Ilavská, S. - Horváthová, M. - Horák, Daniel
Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity.
Croatian Medical Journal. Roč. 57, č. 2 (2016), s. 165-178. ISSN 0353-9504. E-ISSN 1332-8166
R&D Projects: GA ČR(CZ) GC16-01128J
Institutional support: RVO:61389013
Keywords : core-shell maghemite nanoparticles * proliferative activity of lymphocytes * phagocytic activity
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 1.619, year: 2016
http://www.cmj.hr/2016/57/2/27106358.htm

To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles.
Permanent Link: http://hdl.handle.net/11104/0258835