Yeast Hosts for the Production of Recombinant Laccases: A Review

0458428 - FGÚ 2017 RIV US eng J - Journal Article
Antošová, Zuzana - Sychrová, Hana
Yeast Hosts for the Production of Recombinant Laccases: A Review.
Molecular Biotechnology. Roč. 58, č. 2 (2016), s. 93-116. ISSN 1073-6085. E-ISSN 1559-0305
R&D Projects: GA TA ČR(CZ) TA01011461
Institutional support: RVO:67985823
Keywords : laccase * yeasts * heterologous expression * recombinant * expression optimization
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.634, year: 2016

Laccases are multi-copper oxidoreductases which catalyze the oxidation of a wide range of substrates during the simultaneous reduction of oxygen to water. These enzymes, originally found in fungi, plants, and other natural sources, have many industrial and biotechnological applications. They are used in the food, textile, pulp, and paper industries, as well as for bioremediation purposes. Although natural hosts can provide relatively high levels of active laccases after production optimization, heterologous expression can bring, moreover, engineered enzymes with desired properties, such as different substrate specificity or improved stability. Hence, diverse hosts suitable for laccase production are reviewed here, while the greatest emphasis is placed on yeasts which are commonly used for industrial production of various proteins. Different approaches to optimize the laccase expression and activity are also discussed in detail here.
Permanent Link: http://hdl.handle.net/11104/0258707